|
主要论著
以第一作者或通讯作者在包括Nature Chemical Biology、Nature Synthesis、Nature Communications等期刊发表论文200余篇，主编出版6部中英文著作和教材。
代表性著作/教材：
1、Liu Long, Chen Jian. 《Systems and Synthetic Biotechnology for Production of Nutraceuticals》(Springer-Nature出版社)，2019年, ISBN: 978-981-15-0445-7
2、Liu Long, Du Guocheng, Liu Yanfeng. 《Systems and Synthetic Metabolic Engineering》(Elsevier出版社). 2020年. ISBN: 978-0-12-821753-5
3、堵国成、刘龙、宋航. 《生物工艺学》.(科学出版社). 2021年. ISBN: 978-7-04-049006-0
4、刘龙、陈坚. 《枯草芽孢杆菌细胞工厂创制及应用》.(科学出版社). 2022年. ISBN: 978-7-03-000000-0
5、刘龙、陈坚、吕雪芹. 《新一代食品生物技术》.(科学出版社). 2024年. ISBN: 978-7-03-077831-4
代表性论文：
1、Cheng Yang, Yu Wenwen, Bi Xinyu, Liu Yanfeng, Li Jianghua, Du Guocheng, Chen Jian, Lv Xueqin, Liu Long*. CarveAdornCurate: A versatile cloud-based platform for constructing multiscale metabolic models. Trends in Biotechnology. 2025. DOI: 10.1016/j.tibtech.2025.01.011.
2、Li Yang, Wu Yaokang, Xu Xianhao, Liu Yanfeng, Li Jianghua, Du Guocheng, Lv Xueqin, Li Yangyang, Liu Long*. A cross-species inducible system for enhanced protein expression and multiplexed metabolic pathway fine-tuning in bacteria. Nucleic Acids Research. 2025. 53: gkae1315.
3、Xu Xianhao, Lv Xueqin, Liu Yanfeng, Li Jianghua, Du Guocheng, Chen Jian, Ledesma-Amaro Rodrigo, Liu Long*. CRISPR/Cas13X-assisted programmable and multiplexed translation regulation for controlled biosynthesis. Nucleic Acids Research. 2025. 53: gkae1293.
4、Yu Wenwen, Jin Ke, Wang Dandan, Wang Nankai, Li Yangyang, Liu Yanfeng, Li Jianghua, Du Guocheng, Lv Xueqin, Chen Jian, Ledesma-Amaro Rodrigo, Liu Long*. De novo engineering of programmable and multi-functional biomolecular condensates for controlled biosynthesis. Nature Communications. 2024. 15: 7989.
5、Bi Xinyu, Cheng Yang, Lv Xueqin, Liu Yanfeng, Li Jianghua, Du Guocheng, Chen Jian, Liu Long*. A multi-omics, machine learning-aware, genome-wide metabolic model of Bacillus subtilis refines the gene expression and cell growth prediction. Advanced Science. 2024. 2408705.
6、Liu Long, Chen Jian*. Engineered yeast brews plant hormones. Nature Synthesis. 2024. 3: 152–153 (News & Views).
7、Wu Yaokang, Li Yang, Liu Yanfeng, Xiu Xiang, Liu Jiaheng, Zhang Linpei, Li Jianghua, Du Guocheng, Lv Xueqin, Chen Jian, Ledesma-Amaro Rodrigo, Liu Long*. Multiplexed in-situ mutagenesis driven by a dCas12a-based dual-function base editor. Nucleic Acids Research. 2024. 53：4739-4755.
8、Lu Jiangong, Lv Xueqin, Yu Wenwen, Zhang Jianing, Lu Jianxing, Liu Yanfeng, Li Jianghua, Du Guocheng, Chen Jian, Liu Long*. Reshaping phosphatase substrate preference for controlled biosynthesis using a “Design–Build–Test–Learn” framework. Advanced Science. 2024. 2309852.
9、Xu Xianhao, Lv Xueqin, Bi Xinyu, Chen Jian, Liu Long*. Genetic circuits for metabolic flux optimization. Trends in Microbiology. 2024. 32: 791-806.
10、Tian Rongzhen, Zhao Runzhi, Guo Haoyu, Yan Kun, Wang Chenyun, Lu Cheng, Lv Xueqin, Li Jianghua, Liu Long, Du Guocheng, Chen Jian, Liu Yanfeng*. Engineered bacterial orthogonal DNA replication system for continuous evolution. Nature Chemical Biology. 2023. 19: 1504-1512.
11、Wu Yaokang, Li Yang, Jin Ke, Zhang Linpei, Li Jianghua, Liu Yanfeng, Du Guocheng, Lv Xueqin, Chen Jian, Ledesma-Amaro Rodrigo, Liu Long*. CRISPR-dCas12a-mediated genetic circuit cascades for multiplexed pathway optimization. Nature Chemical Biology. 2023. 19: 367-377.
12、Xu Xianhao, Lv Xueqin, Cui Shixiu, Liu Yanfeng, Xia Hongzhi, Li Jianghua, Du Guocheng, Li Zhaofeng, Rodrigo Ledesma-Amaro, Chen Jian, Liu Long*. Remodeling isoprene pyrophosphate metabolism for promoting terpenoids bioproduction. Engineering. 2023. 28: 166-178.
13、Sun Guoyun, Wu Yaokang, Huang Ziyang, Liu Yanfeng, Li Jianghua, Du Guocheng, Lv Xueqin, Liu Long*. Directed evolution of diacetylchitobiose deacetylase via high-throughput droplet sorting with a novel, bacteria-based biosensor. Biosensors and Bioelectronics. 2023. 219: 114818.
14、Yu Wenwen, Jin Ke, Wu Yaokang, Zhang Quanwei, Liu Yanfeng, Li Jianghua, Du Guocheng, Chen Jian, Lv Xueqin, Ledesma-Amaro Rodrigo, Liu Long*. A pathway independent multi-modular ordered control system based on thermosensors and CRISPRi improves bioproduction in Bacillus subtilis. Nucleic Acids Research. 2022. 50: 6587-6600.
15、Xu Yameng, Wang Xinglong, Zhang Chenyang, Zhou Xuan, Xu Xianhao, Han Luyao, Lv Xueqin, Liu Yanfeng, Liu Song, Li Jianghua, Du Guocheng, Chen Jian, Ledesma-Amaro Rodrigo, Liu Long*. De novo biosynthesis of rubusoside and rebaudiosides in engineered yeasts. Nature Communications. 2022. 13: 3040.
16、Jiang Wei, Villamor David Hernandez, Peng Huadong, Chen Jian, Liu Long, Haritos Victoria, Ledesma-Amaro Rodrigo. Metabolic engineering strategies to enable microbial utilization of C1 feedstocks. Nature Chemical Biology. 2021. 17: 845-855.
17、Xu Xianhao, Li Xueliang, Liu Yanfeng, Zhu Yonglian, Li Jianghua, Du Guocheng, Chen Jian, Ledesma- Amaro Rodrigo, Liu Long*. Pyruvate-responsive genetic circuits for dynamic control of central metabolism. Nature Chemical Biology. 2020. 16: 1261-1268.
18、Tian Rongzhen, Liu Yanfeng, Cao Yanting, Zhang Zhongjie, Li Jianghua, Liu Long, Du Guocheng, Liu Yanfeng*, Chen Jian*. Titrating bacterial growth and chemical biosynthesis for efficient N-acetylglucosamine and N-acetylneuraminic acid bioproduction. Nature Communications. 2020. 11: 5078.
19、Wu Yaokang, Chen Taichi, Liu Yanfeng, Tian Rongzhen, Lv Xueqin, Li Jianghua, Du Guocheng, Chen Jian, Ledesma-Amaro Rodrigo, Liu Long*. Design of a programmable biosensor-CRISPRi genetic circuits for dynamic and autonomous dual-control of metabolic flux in B. subtilis. Nucleic Acids Research. 2020. 48: 996-1009.
20、Liu Yanfeng, Link Hannes, Liu Long*, Du Guocheng, Chen Jian*, Sauer Uwe. A novel dynamic pathway analysis approach reveals a limiting futile cycle in N-acetylglucosamine overproducing Bacillus subtilis. Nature Communications. 2016. 7:11933. |
|
以第一发明人获得授权发明专利103项，其中美国授权发明专利19项。部分授权发明专利如下：
1. Liu Long, Lv Xueqin, Du Guocheng, Li Jianghua, Chen Jian. Recombinant Bacillus subtills and use thereof. 授权专利号：US 11,136,545 B2
2. Liu Long, Chen Jian, Du Guocheng, Lv Xueqin, Li Jianghua, Jiang Zhu, Lu Wei, Zhang Hongzhi, Lu Jianxing, Liu Changfeng. Construction and application of engineering bacteria capable of secreting and expressing diacetylchitobiose deacetylase. 授权专利号：US 10,934,552 B2
3. Liu Long, Chen Jian, Du Guocheng, Li Jianghua, Wu Yaokang, Chen Taichi. Method for producing N-acetylglucosamine by co-utilizing glucose and xylose based on CRISPRi. 授权专利号：US 10,633,658 B2
4. Liu Long, Chen Jian, Du Guocheng, Yin Xian, Li Jianghua. Transgenic strain containing an isolated promoter. 授权专利号：US 10,669,550 B2
5. Liu Long, Gu Yang, Deng Jieying, Chen Jian, Du Guocheng, Li Jianghua. Recombinant strain of Bacillus subtilis. 授权专利号：US 10,669,532 B2
6. Liu Long, Li Jianghua, Niu Tengfei, Chen Jian, Du Guocheng. Recombinant Bacillus subtilis for increasing production of acetylglucosamine and construction method thereof. 授权专利号：US 10,465,198 B2
7. Liu Long, Chen Jian, Du Guocheng, Li Jianghua, Niu Tengfei. Recombinant Bacillus subtilis for producing acetylglucosamine and construction method thereof. 授权专利号：US 10,093,936 B2
8. Liu Long, Chen Jian, Du Guocheng, Yin Xian, Li Jianghua. Method of gene expression regulation. 授权专利号：US 2018/0171344 A1
9. Liu Long, Chen Jian, Du Guocheng, Li Jianghua, Liu Yanfeng, Hannes Link, Uwe Sauer. Method for enhancing N-acetylglucosamine production through glck knockout of Bacillus subtilis. 授权专利号：US 9,914,949 B2
10. Liu Long, Chen Jian, Du Guocheng, Yin Xian, Li Jianghua. Promoter and use thereof. 授权专利号：US 9,926,569 B2. |
当前位置:
