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酶/功能蛋白挖掘与改造
1、 Zeng B, Ouyang S, Wang ZX, Cui SX, Du GC* and Liu S*. Ancestral bacterial laccase enables efficient biodetoxification of inhibitory wheat straw hydrolysate for sustainable microbial protein production. Green Chemistry. 2026, 28: 2958-2971.
2、 Zeng B, Fu YS, Ye JC, Yang PH, Cui SX, Qiu WX, Li YY, Wu TX, Zhang HY, Wang YC, Du GC*, Liu S*. Ancestral sequence reconstruction of the prokaryotic three-domain laccases for efficiently degrading polyethylene. Journal of Hazardous Materials. 2024, 476: 135012.
3、 Fu YS, Li YY, Pan F, Aqeel SM, Liu S*, Chen J*. Rational engineering of an antifreeze-based biomaterial with dual-enhanced ice-binding and delivery for high-efficiency cryopreservation. Journal of Advanced Research. 2025, doi.org/10.1016/j.jare.2025.12.008.
4、 Fu YS, Li YY, Liu S*, Chen J*. The expanding horizons of antifreeze proteins from food science to multidisciplinary technological innovations. Trends in Food Science & Technology. 2025, 164: 105252.
5、 Qiu WX, Yang PH, Ye JC, Zhou JW, and Liu S*. Unveiling highly active and stable L‑glutaminase through ancestral sequence reconstruction and turnover number prediction. Journal of Agricultural and Food Chemistry. 2025, 73: 5353-5362.
6、 Tan YM, Yang PH, Qiu WX, Xue HJ, Du GC, Zhou JW, and Liu S*. Thermostability enhancement of Streptomyces mobaraensis transglutaminase by folding free energy-guided helix-corner motif engineering. Journal of Agricultural and Food Chemistry. 2026, 74(11): 9655-9665.
7、 Tan YM, Yang PH, Qiu WX, Ye JC, and Liu S*. Enhanced thermostability of Streptomyces mobaraenesis transglutaminase by multirational design methods. Journal of Agricultural and Food Chemistry. 2025, 73: 29797-29807.
8、 Yang PH, Wang XL, Ye JC, Rao SQ, Zhou JW, Du GC, Liu S*. Enhanced thermostability and catalytic activity of Streptomyces mobaraenesis transglutaminase by rationally engineering its flexible regions. Journal of Agricultural and Food Chemistry. 2023, 71(16): 6366-6375.
9、 Wang XL, Du JH, Zhao BC, Wang H, Rao SQ, Du GC, Zhou JW, Chen J *, Liu S*. Significantly improving the thermostability and catalytic efficiency of Streptomyces mobaraenesis transglutaminase through combined rational design. Journal of Agricultural and Food Chemistry. 2021, 69(50): 15268-15278.
10、 Song WY, Li YY, Tong Y, Li Y, Tao J, Rao SQ, Li JH, Zhou JW, Liu S*. Improving the catalytic efficiency of Aspergillus fumigatus glucoamylase toward raw starch by engineering its N‑glycosylation sites and saturation mutation. Journal of Agricultural and Food Chemistry. 2022, 70: 12672-12680.
11、 Li YY, Zhang HY, Fu YS, Zhou ZT, Yu WW, Zhou JW, Li JH, Du GC, Liu S*. Enhancing acid resistance of Aspergillus niger pectin lyase through surface charge design for improved application in juice clariffcation. Journal of Agricultural and Food Chemistry. 2024, 72(20): 11652-11662.
12、 Li YY, Li C, Huang H, Rao SQ, Zhang Q, Zhou JW, Li JH, Du GC, Liu S*. Significantly enhanced thermostability of Aspergillus niger xylanase by modifying its highly flexible regions. Journal of Agricultural and Food Chemistry. 2022, 70 (15): 4620-4630.
黑曲霉
13、 Li YY, Li C, Aqeel SM, Wang YC, Zhang Q, Ma JL, Zhou JW, Li JH, Du GC, Liu S*. Enhanced expression of xylanase in Aspergillus niger enabling a two-step enzymatic pathway for extracting β-glucan from oat bran. Bioresource Technology. 2023, 377: 128962.
14、 Li YY, Song WY, Han XY, Wang YC, Rao SQ, Zhang Q, Zhou JW, Li JH, Liu S*, Du GC. Recent progress in key lignocellulosic enzymes: Enzyme discovery, molecular modifications, production, and enzymatic biomass saccharification. Bioresource Technology. 2022, 363: 127986.
15、 Li YY, Li C, Fu YS, Zhang Q, Ma JN, Zhou JW, Li JH, Du GC, Liu S*. A CRISPR/Cas9-based visual toolkit enabling multiplex integration at specific genomic loci in Aspergillus niger. Synthetic and Systems Biotechnology. 2024, 9(2): 209-216.
16、 Li C, Zhou JW, Rao SQ, Du GC*, Liu S*. Visualized multigene editing system for Aspergillus niger. ACS Synthetic Biology. 2021, 10(10): 2607-2616.
17、 Li C, Zhou JW, Du GC ⁎, Chen J, Takahashi S, Liu S ⁎. Developing Aspergillus niger as a cell factory for food enzyme production. Biotechnology Advances. 2020, 44: 107630.
茂原链霉菌
18、 Xue HJ, Zhou JW, Du GC, Liu S*. Heterologous expression of phospholipase A1 in Streptomyces mobaraensis and its application in crude oil degumming. Process Biochemistry. 2026, 163: 114-121
19、 Ye JC, Yang PH, Zhou JW, Du GC, Liu S*. Efficient production of a thermostable mutant of transglutaminase by Streptomyces mobaraensis. Journal of Agricultural and Food Chemistry. 2024, 72(8): 4207-4216.
20、 Yin XQ, Li YY, Zhou JW, Rao SQ, Du GC, Chen J*, Liu S*. Enhanced production of transglutaminase in Streptomyces mobaraensis through random mutagenesis and site-directed genetic modification. Journal of Agricultural and Food Chemistry. 2021, 69(10): 3144-3153.
枯草芽孢杆菌
21、 Xu KD, Tong Y, Li Y, Tao J, Rao SQ, Li JH, Zhou JW, Liu S*. Autoinduction AND gate inhibits cell lysis to enhance protein production in Bacillus subtilis controlled by population density and cell physiological state. ACS Synthetic Biology. 2023, 12(3): 842-851.
22、 Xu KD, Tong Y, Li Y, Tao J, Rao SQ, Li JH, Zhou JW, Liu S*. Autoinduction expression modules for regulating gene expression in Bacillus subtilis. ACS Synthetic Biology. 2022, 11(12), 4220-4225
23、 Xu KD, Tong Y, Li Y, Tao J, Rao SQ, Li JH*, Zhou JW, Liu S*. Efficient, flexible autoinduction expression systems with broad initiation in Bacillus subtilis. ACS Synthetic Biology. 2021, 10(11): 3084-3093.
24、 Xu KD, Tong Y, Li Y, Tao J, Li JH*, Zhou JW, Liu S*. Rational design of the N-terminal coding sequence for regulating enzyme expression in Bacillus subtilis. ACS Synthetic Biology. 2021, 10(2): 265-276.
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